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  • Title: Isolation of amine oxidase from bovine plasma by a two-step procedure.
    Author: Vianello F, Di Paolo ML, Zennaro L, Stevanato R, Rigo A.
    Journal: Protein Expr Purif; 1992 Oct; 3(5):362-7. PubMed ID: 1333859.
    Abstract:
    A novel method for isolation of amine oxidase from bovine plasma is reported; it involves a two-step procedure, namely ammonium sulfate fractionation and affinity chromatography with elution by aniline, which is a competitive inhibitor of the enzyme. A homogeneous enzyme, characterized by a specific activity of 0.44 U/mg, was obtained with a yield higher than 50%.
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