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  • Title: The alpha antitoxin content of the international reference preparations of Clostridium welchii types B and D antitoxins.
    Author: SKULBERG A, VAN HEYNINGEN WE.
    Journal: Bull World Health Organ; 1956; 14(3):557-66. PubMed ID: 13342930.
    Abstract:
    The alpha antitoxin contents of the International Reference Preparations of Clostridium welchii Types B and D Antitoxins were assayed against two type A test toxins-Cl. welchii (perfringens) test toxin P2, issued by the Statens Seruminstitut, Copenhagen, and Cl. welchii test toxin AGX 1373, issued by the Wellcome Research Laboratories, England-the alpha toxin contents of which were first determined against the International Standard Alpha Antitoxin. Three methods of assay were used: mouse killing (the L+ test); haemolysis of red blood cells (the Lh test); and production of turbidity in egg-yolk solution (the lecithinase or Lv test).Taking the mean of all tests, it was found that the International Reference Preparation of Cl. welchii Type B Antitoxin contained 282 units of alpha antitoxin per ml, while the International Reference Preparation of Cl. welchii Type D Antitoxin contained 93 units of alpha antitoxin per ml.
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