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  • Title: [Use of non-radioactive biotin-labelled probes for detecting hepatitis A virus].
    Author: Budiak EV, Safronov IV, Baturina II, Sinagatullina NM, Naumov VA, Khomiakov AG, Shishkin GV, Oreshkova SF, Veĭko NN, Karpukhin AV.
    Journal: Mol Gen Mikrobiol Virusol; 1992; (9-10):5-8. PubMed ID: 1338551.
    Abstract:
    The biotin-labeled DNA probes were constructed on the basis of the hybrid bacteriophage M13nip 9 single-stranded DNA containing the fragments of the hepatitis A viral cDNA. The probes were biotin treated by chemical modification of the DNA by the peraminating reagent or photochemically. The labeled DNA probes were used in molecular hybridization experiments with the nuclear acids fixed on the nitrocellulose filters. The biotin treated DNA was determined by the avidin-gold colloid conjugate with the subsequent physical silver amplification or by the streptavidin-alkaline phosphatase conjugate. The sensitivity of both probes was identical and permitted the determination of 5 x 10(-11)-5 x 10(-12) g of the control DNA and 10(-9) g of the hepatitis A virus. The developed test systems were used for detection of the viral RNA in blood from patients.
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