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  • Title: [Interleukin 6 production and interleukin 6 mRNA gene expression by mouse glomerular mesangial cells].
    Author: Chen XM.
    Journal: Zhonghua Yi Xue Za Zhi; 1992 Nov; 72(11):664-6, 702. PubMed ID: 1338703.
    Abstract:
    To investigate the relationship between Interleukin 6(IL 6) and glomerular mesangial cells (MC), the MC of BALB/c mouse were cultured and verified subsequently by anti-keratin and antidesmin antibodies. The cultured MC were divided into two parts those with Mac-1 marker and those without by flow cytometry. The IL 6 activity of cultured supernatants of MC was measured using the IL 6-dependent cell line subclone (MH 60). The results show that the mouse MC can produce IL6 and the level of IL 6 produced by MC with Mac-1 marker was higher than that without Mac-1. The RNA from the mouse glomerular mesangial cells was hybridized in dot blots with a-32pdCTP labeled IL6 cDNA probe, the dot blot autoradiograph showed the expression of IL6 mRNA gene in mouse mesangial cells.
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