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  • Title: Characterization of the 5'-flanking region of the human brain-derived neurotrophic factor gene.
    Author: Shintani A, Ono Y, Kaisho Y, Igarashi K.
    Journal: Biochem Biophys Res Commun; 1992 Jan 15; 182(1):325-32. PubMed ID: 1339267.
    Abstract:
    The 5'-flanking region of the human brain-derived neurotrophic factor (BDNF) gene was isolated from a human placental genomic library using the cDNA fragment for the 5'-noncoding region of human BDNF as a probe. A 3.2 Kbp genomic fragment containing the 5'-flanking region, the first exon and a portion of the first intron was isolated and sequenced. The transcriptional initiation site, identified by S1 nuclease mapping, was located 26 bp downstream from the TATA-like sequence. Several expression plasmids, in which the BDNF promoter regions were fused to the chloramphenicol acetyltransferase (CAT) gene, were constructed. Transient expression in human glioma Hs683 cells demonstrated that a fragment of about 0.5 Kbp from the transcriptional initiation site was sufficient for promoter activity.
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