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  • Title: The identification of an eosinophil differentiation factor in culture supernatant of mononuclear cells from asthmatics.
    Author: Tsai JJ, Yu LN, Su SN, Wang SR.
    Journal: Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi; 1992 Aug; 25(3):196-207. PubMed ID: 1342005.
    Abstract:
    Although peripheral eosinophilia is a common feature of bronchial asthma, the precise mechanism of its production is still unknown. It has recently been reported that murine interleukin-5 (IL-5) can cross-react with human cells and selectively stimulates the proliferation and differentiation of eosinophils. This study identified the IL-5-like activity in culture supernatants of peripheral blood mononuclear cells (MNCs) from asthmatic patients. Murine recombinant IL-5 (rIL-5) was used as a positive control; the number of eosinophils was determined by both Wright's stain and eosinophil peroxidase measurement. Time course and dose response studies showed that rIL-5 at 40 U/ml induced a maximal eosinophil differentiation after a three week incubation with cord blood MNCs. Unstimulated MNC supernatants obtained from asthmatics possessed a higher eosinophil differentiation activity (OD490, 0.09 +/- 0.02, n = 23) than those obtained from the normals (0.03 +/- 0.01) (P < 0.02). This activity in unstimulated MNC supernatant can be neutralized by anti-IL-5 antibodies. Neither Bermuda grass- nor phytohemagglutinin-stimulated MNC supernatant showed a statistical significance between these two groups. The IL-5-like activity was associated with a protein of MW around 30kD as determined by Superose-12 PG gel filtration. In conclusion, MNC culture supernatants derived from asthmatics contained an eosinophil differentiation activity, which might be important for regulation of eosinophil generation and thus contribute to the asthma-related eosinophilia.
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