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  • Title: Purification and characterization of an agglutinin of the soft coral Sinularia species.
    Author: Goto R, Muramoto K, Yamazaki M, Kamiya H.
    Journal: Dev Comp Immunol; 1992; 16(1):9-17. PubMed ID: 1352264.
    Abstract:
    A D-galactose-specific agglutinin, named sinularian, has been isolated from the soft coral Sinularia sp. by affinity chromatography on acid-treated Sepharose 4B and by gel filtration on HPLC. Sinularian was a glycoprotein containing 11% sugar. It gave a single band corresponding to 78 kDa in SDS-PAGE, irrespective of a treatment with 2-mercaptoethanol. Sinularian agglutinated rabbit erythrocytes and murine leukemia cells but not sheep or human ABO erythrocytes. Its hemagglutinating activity was Ca(++)-independent. Sinularian promoted binding of macrophages to tumor cells.
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