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  • Title: Improvement in HLA-DQB typing by PCR-RFLP: introduction of a constant restriction site in one of the primers for digestion control.
    Author: Mercier B, Ferec C, Dufosse F, Huart JJ.
    Journal: Tissue Antigens; 1992 Aug; 40(2):86-9. PubMed ID: 1357779.
    Abstract:
    The PCR-RFLP method previously reported by Inoko is a powerful technique for HLA class II typing. The reliability of RFLP interpretation depends on complete digestion by restriction endonucleases using a modified primer with restriction sites as an internal digestion control. The use of restriction enzymes which recognize specific HLA DQB allelic variations makes HLA DQB genotyping possible.
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