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  • Title: Purification of testicular transglutaminase by hydrophobic chromatography on phenyl-sepharose.
    Author: Bergamini CM, Signorini M.
    Journal: Biochem Int; 1992 Aug; 27(4):557-65. PubMed ID: 1358070.
    Abstract:
    We developed a procedure for purification to homogeneity of cytosolic transglutaminase from bovine testes, by means of anion exchange, size exclusion and hydrophobic chromatography. The crucial step is chromatography on Phenyl sepharose, with specific elution by the inhibitor GTP. The enzyme displays the properties of type 2 transglutaminases.
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