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  • Title: Purification of Rhodotorula gracilis D-amino acid oxidase.
    Author: Pollegioni L, Pilone MS.
    Journal: Protein Expr Purif; 1992 Apr; 3(2):165-7. PubMed ID: 1358302.
    Abstract:
    A protocol is presented for preparing Rhodotorula gracilis D-amino acid oxidase in homogeneous form and in high yield in 3 to 4 days. The method takes advantage of (a) cell rupture by alternate freeze-thawing, (b) use of DEAE-Sepharose to bind contaminants, and (c) enzyme binding to a Mono S column. The D-amino acid oxidase isolated by this means has the same spectral and catalytic properties as the enzyme previously obtained, and possesses improved long-term stability.
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