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  • Title: Kinetics and properties of L-glutaminase and L-asparaginase activities of Pseudomonas ovalis.
    Author: Badr El-Din SM, Foda MS.
    Journal: Zentralbl Bakteriol Parasitenkd Infektionskr Hyg; 1976; 131(3):489-96. PubMed ID: 13588.
    Abstract:
    Pseudomonas ovalis produces L-glutaminase and L-asparaginase activities simultaneously upon induction by L-glutamine or L-asparagin in the growth medium. Both activities are confined to the cell during active growth and are not released into the medium. The apparent Km values are 1.4 X 10(-2) M and 6 X 10(-3) M for L-glutamine and L-asparagine substrates, respectively. Induction of both activities is substantially favoured in media with initial pH values higher than 7. In buffered yeast extract L-asparagine medium, significant amounts of L-glutaminase and L-asparaginase activities appeared towards the end of the exponential phase and along the stationary phase. The process of enzyme formation showed a firm link to the cell active growth, as evidenced by the use of growth inhibitors.
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