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  • Title: Production of Neisseria gonorrhoeae pili (fimbriae) in Pseudomonas aeruginosa.
    Author: Hoyne PA, Haas R, Meyer TF, Davies JK, Elleman TC.
    Journal: J Bacteriol; 1992 Nov; 174(22):7321-7. PubMed ID: 1358873.
    Abstract:
    Pseudomonas aeruginosa K/2PfS, when transformed with an expression plasmid harboring the pilin gene (pilE1) of Neisseria gonorrhoeae MS11, was able to express and assemble gonococcal pilin monomers into surface-associated pili, as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, immunoblotting, and immunoelectron microscopy. Concomitant with the expression of gonococcal pili in P. aeruginosa was the virtual loss of production of P. aeruginosa K/2PfS pili normally associated with the host cell.
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