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  • Title: [Soluble metabolic antigens of Trichinella spiralis: their isolation and characteristics].
    Author: Kostetskiĭ AF, Vaserin IuI.
    Journal: Parazitologiia; 1992; 26(1):62-6. PubMed ID: 1364527.
    Abstract:
    Cultivation of Trichinella muscular larvae, purified by centrifugation in 20 ... 50% saccharose density gradient, in protein--free nutrient media at a dosage of 3.5-.10(3) lar./ml in the presence of insulin has made it possible to obtain a soluble antigen of Trichinella. It has been shown by means of electrophoresis in polyacrylamide gel that the soluble (secretory-excretory) antigen has three protein fractions while the somatic Trichinella antigen has 18 fractions. It has been shown that the soluble (secretory-excretory) antigen can be used for immobilization of erythrocytes on the surface that enables the sensitivity and specificity of serological methods for diagnosis of trichinellosis to be increased.
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