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  • Title: Mechanical stability and diffusional resistance of a polymeric gel used for biocatalyst immobilization.
    Author: de Alteriis E, Scardi V, Masi P, Parascandola P.
    Journal: Enzyme Microb Technol; 1990 Jul; 12(7):539-45. PubMed ID: 1366636.
    Abstract:
    The mechanical strength of gelatin gels insolubilized by crosslinking with formaldehyde was measured at various gelatin percentages and formaldehyde-to-gelatin ratios. This property was shown to be related to the characteristic sponge-like structure of the insolubilized gelatin gel, a structure that unexpectedly is also responsible for the resistance to substrate and product diffusion. A comparison between immobilizates of invertase and invertase-active yeast cells prepared with different gelatin concentrations showed that the enzyme, in contrast to cells, is deeply involved in the gel insolubilization process. The catalytic behavior of agar, kappa-carrageenan, alginate, and gelatin immobilizates was compared under the same conditions of cell loading.
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