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  • Title: Cloning of a gene coding for phosphotransacetylase from Escherichia coli.
    Author: Yamamoto-Otake H, Matsuyama A, Nakano E.
    Journal: Appl Microbiol Biotechnol; 1990 Sep; 33(6):680-2. PubMed ID: 1366944.
    Abstract:
    A phosphotransacetylase gene (pta) has been cloned from a genomic DNA library of Escherichia coli 1100, a derivative of strain K-12. The phosphotransacetylase activities of pta+ plasmid-containing strains were amplified about 150-fold under control of the lac promoter. The molecular weight of the phosphotransacetylase was estimated to be about 81,000 by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. The pta gene was found to be downstream of ackA by a combination of restriction analysis and plasmid subcloning. It is located about 13 kb upstream of the purF-folC-hisT region of the chromosome.
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