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  • Title: Pilot scale production of a Trichoderma reesei endo-beta-glucanase by brewer's yeast.
    Author: Zurbriggen BD, Penttilä ME, Viikari L, Bailey MJ.
    Journal: J Biotechnol; 1991 Feb; 17(2):133-46. PubMed ID: 1366983.
    Abstract:
    Endo-beta-glucanase I (EGI) of Trichoderma reesei was produced in laboratory and pilot scale using recombinant strains of "bottom-fermenting" Saccharomyces cerevisiae. The gene eg/1 was integrated in the chromosome or an expression cassette was inserted on a multicopy plasmid. Expression levels were compared in a laboratory scale bioreactor. The best EGI-producing strain was cultivated in pilot scale. Adsorbent treatment was used to remove endogenous yeast proteins and other impurities from the culture filtrate during concentration. Effective pilot scale one-step purification of the EGI protein was obtained using DEAE-Sepharose, on which EGI was weakly bound. The purified enzyme reacted with antibodies prepared against T. reesei EGI and catalyzed the hydrolysis of both insoluble and soluble substrates.
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