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  • Title: Extracellular production of human tumor necrosis factor-alpha by Escherichia coli using a chemically-synthesized gene.
    Author: Nakamura S, Masegi T, Kitai K, Ichikawa Y, Kudo T, Aono R, Horikoshi K.
    Journal: Agric Biol Chem; 1990 Dec; 54(12):3241-50. PubMed ID: 1368641.
    Abstract:
    A DNA fragment of approximately 490 base pairs encoding human TNF was chemically synthesized and expressed within Escherichia coli cells. Furthermore, extracellular production of human TNF and several N-terminal deletion mutants of TNF was attempted using the excretion vector pEAP8. The TNF mutant with two N-terminal amino acids deleted (N delta 2-TNF) was efficiently excreted into the culture medium by E. coli carrying the plasmid pEXTNF3. In this clone, the signal peptide was correctly processed during the excretion. The E. coli-excreted N delta 2-TNF had higher antitumor activity than wild-type TNF or N delta 2-TNF produced intracellularly by E. coli.
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