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  • Title: Continuous production of large amounts of monoclonal immunoglobulins in hollow fibers using protein-free medium.
    Author: Dhainaut F, Bihoreau N, Meterreau JL, Lirochon J, Vincentelli R, Mignot G.
    Journal: Cytotechnology; 1992; 10(1):33-41. PubMed ID: 1369103.
    Abstract:
    The performance of a protein-free medium was compared in culture flasks with a serum-supplemented medium and with a serum free medium in terms of cell growth and monoclonal antibody production by a murine hybridoma. We present results of continuous production in hollow fiber culture systems using serum-free medium and protein-free medium. In protein-free medium, it has been possible to produce large quantities of monoclonal antibody with a productivity similar to that obtained in serum-free medium. After a two steps purification process, monoclonal antibodies were characterized by SDS-PAGE, High Performance Size Exclusion Chromatography and Free Solution Capillary Electrophoresis. SDS-PAGE and high performance chromatography analysis have showed that purified monoclonal antibodies produced in serum-free medium or protein-free medium were similar. Furthermore, Capillary Electrophoresis characterization revealed that both MAbs were constituted by three isoforms with equivalent electrophoretic mobilities.
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