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  • Title: Interactions between novel tumor necrosis factor-alpha mutants and receptors on tumor and normal cells.
    Author: Nakamura S, Masegi T, Fukuoka M, Kitai K, Kato A, Ichikawa Y, Watanabe N, Niitsu Y.
    Journal: Agric Biol Chem; 1991 Jan; 55(1):53-7. PubMed ID: 1369315.
    Abstract:
    We prepared several TNF mutants by protein engineering techniques and compared their biological properties with those of the wild-type TNF. The mutant that lacked 7 N-terminal amino acids had higher cytotoxicity and higher binding activity to receptors on tumor cells. In contrast, the mutagenesis of Arg32 or Ala84, in combination with the deletion of 7 N-terminal amino acids, eliminated the cytotoxicity and the receptor binding. These mutants also lacked acute lethal toxicity in normal mice. Therefore, we concluded that the N-terminus, Arg32 and Ala84 of TNF might be concerned with binding to the TNF receptor. It was also suggested that the receptor molecules on tumor cells bound to the same or neighboring sites on TNF molecules as normal cell receptors.
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