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  • Title: Characterization of Shiga-like toxin producing Escherichia coli (SLTEC) isolated from calves with and without diarrhoea.
    Author: Wieler LH, Bauerfeind R, Baljer G.
    Journal: Zentralbl Bakteriol; 1992 Jan; 276(2):243-53. PubMed ID: 1373093.
    Abstract:
    To determine if shiga-like toxin producing Escherichia coli (SLTEC) are involved in neonatal calf diarrhoea, isolated E. coli strains from diarrhoeic and non-diarrhoeic calves were characterized for shiga-like toxin (SLT) by colony blot hybridization and cytotoxicity assays. None of 150 E. coli strains isolated from diarrhoeic calves in 1985-1988 was positive for SLT, while 7/232 (3.0%) isolated in 1989 were positive for SLT. In contrast, samples collected during 1989 and 1990 from diarrhoeic calves were 21.9% SLTEC positive, and samples from non-diarrhoeic calves were 12.9% SLTEC positive. SLT I positive E. coli strains were isolated more often from diseased (17.8%) than from healthy animals (5.0%), while SLT II positive E. coli were more often detected in non-diarrhoeic (8.9%) than in diarrhoeic calves (4.1%). The mean percentage of SLT I positive E. coli in the whole E. coli flora of the samples was significantly higher in diarrhoeic than in healthy animals, implying a pathogenic role of SLT I producing E. coli in neonatal calf diarrhoea. Enterohemolysin was produced by 70.8% of the SLT I producing E. coli strains examined. Determination of O- and K-antigens of SLT positive E. coli revealed a highly diverse spectrum of SLTEC O-groups in calves. While no E. coli isolate belonged to serotype O157:H7, classical human enteropathogenic E. coli O-groups (O26, O111, O128) were detected. These results support the theory that cattle serve as a reservoir for human SLTEC infection.
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