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  • Title: Increased resistance to the toxic effects of alkylating agents in tobacco expressing the E. coli DNA repair gene ada.
    Author: Angelis K, Bríza J, Satava J, Skákal I, Velemínský J, Vlasák J, Kleibl K, Margison GP.
    Journal: Mutat Res; 1992 May; 273(3):271-80. PubMed ID: 1374848.
    Abstract:
    The protein coding region of the E. coli gene ada has been transferred to tobacco plants by a leaf disc transformation procedure involving an Agrobacterium tumefaciens Ti plasmid. Transformed plants were shown to be transgenic for the ada message and had increased levels of O6-alkylguanine DNA alkyltransferase activity. The N-methyl-N-nitrosourea- or taurinechlorethylnitrosourea-induced inhibition of growth of calluses or of cells in suspension was considerably lower in ada-transformed than in non-transformed plants. This indicates that O6-alkylguanine, O4-alkylthymine or phosphotriesters are growth-inhibitory lesions in tobacco.
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