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  • Title: Dietary regulation of pancreatic amylase in transgenic mice mediated by a 126-base pair DNA fragment.
    Author: Schmid RM, Meisler MH.
    Journal: Am J Physiol; 1992 Jun; 262(6 Pt 1):G971-6. PubMed ID: 1377451.
    Abstract:
    Expression of the mouse pancreatic amylase gene Amy-2.2 is increased approximately 10-fold in response to increasing the carbohydrate content of the diet from 9.6 to 74%. The DNA sequence mediating this response has been localized to the 5' flanking region of the amylase gene by analysis of hybrid constructs in transgenic mice. The results define a 127-base pair dietary response unit that includes two previously described regulatory elements, an insulin-responsive element and a pancreatic enhancer. Fragments containing these two elements alone fail to respond to diet, demonstrating a requirement for additional regulatory sequences. Another mouse amylase gene Amy-2.1 is only minimally responsive to insulin and to diet. The data are consistent with the hypothesis that the insulin-response element is necessary but not sufficient for regulation of amylase by dietary carbohydrate.
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