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  • Title: In vivo and in vitro interaction between interleukin 6 and granulocyte colony-stimulating factor in the regulation of murine hematopoiesis.
    Author: Pojda Z, Tanaka K, Aoki Y, Tsuboi A.
    Journal: Exp Hematol; 1992 Aug; 20(7):874-8. PubMed ID: 1378408.
    Abstract:
    The interaction both in vitro and in vivo between human recombinant interleukin 6 (IL-6) and human recombinant granulocyte colony-stimulating factor (G-CSF) in the regulation of mouse hematopoiesis was investigated. In the in vitro experiments, mouse bone marrow and spleen cells were cultured in semisolid medium containing 5 or 50 ng/ml of G-CSF and concentrations ranging from 0 to 20 ng/ml of IL-6. In vivo, mice were treated for 4 days with 15, 50, or 250 micrograms/kg body weight/day of G-CSF, or with similar doses of G-CSF plus 50 micrograms/kg/day of IL-6, and the numbers of stem (spleen colony-forming units, CFU-S) and progenitor (megakaryocyte colony-forming cells, Meg-CFC; granulocyte-macrophage colony-forming cells, GM-CFC) hematopoietic cells and mature circulating blood cells were evaluated. In vitro IL-6 caused dose-dependent suppression of the proliferation of GM-CFC, decreasing numbers of granulocyte-macrophage colonies in culture. The inhibitory effect of IL-6 decreased along with the increase of density of cultured cells, suggesting the influence of accessory, cytokine-producing cells. In vivo, the numbers of GM-CFC and Meg-CFC in mice treated with IL-6 plus G-CSF were significantly closer to the values observed in untreated animals than those in mice treated with G-CSF only. The other cell populations were unaffected by IL-6 treatment. Our results demonstrate antagonism between IL-6 and G-CSF in the in vitro stimulation of the proliferation of late granulocyte precursors, and they suggest a similar effect in the in vivo regulation of granulopoiesis and megakaryocytopoiesis at the progenitor cell level.
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