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  • Title: MPM-12: a monoclonal antibody that predominantly stains mitotic cells and recognizes a protein kinase.
    Author: Ganju RK, Penkala JE, Wright DA, Davis FM, Rao PN.
    Journal: Eur J Cell Biol; 1992 Feb; 57(1):124-31. PubMed ID: 1379180.
    Abstract:
    The monoclonal antibody MPM-12, raised by using partially purified extract of mitotic HeLa cells as the immunogen, preferentially stains the cytoplasm of mitotic cells by indirect immunofluorescence without exhibiting any species specificity. On immunoblots, MPM-12 recognizes three bands, of 155, 88, and 68 kDa, in mitotic HeLa cell extract but only the 68-kDa band in interphase cell extract. The 68-kDa band seems to be associated with chromatin while the other two are not. All three MPM-12 reactive peptides are phosphorylated, and the phosphorylation seems to be required for MPM-12 reactivity. The MPM-12 immunocomplexes exhibit autophosphorylating and histone H1 kinase activity.
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