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  • Title: Hemoglobin A1c by HPLC with the Pharmacia Mono S HR 5/N cation-exchange column: influence of sample protein load on optimal chromatographic conditions.
    Author: Philcox JC, Haywood MR, Rofe AM.
    Journal: Clin Chem; 1992 Aug; 38(8 Pt 1):1488-90. PubMed ID: 1379526.
    Abstract:
    The Pharmacia Mono S HR 5/5 column has been optimized for hemoglobin A1c analysis by HPLC by using a much smaller column load, decreased buffer flow rate, and a steeper gradient than was used in previously described methods. Superior chromatographic separation, shorter analysis time, and a greatly extended column life have resulted.
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