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  • Title: Estrogens in maternal plasma following intraamniotic injection of (3H)-dehydroepiandrosterone-sulfate in midpregnancy.
    Author: Lehmann WD, Strecker JR.
    Journal: J Perinat Med; 1976; 4(4):255-60. PubMed ID: 137965.
    Abstract:
    In 4 patients with normal pregnancies between the 18th and 20th week of gestation (3H7alpha)-dehydroepiandrosterone-sulfate ([H]-DHEA-S) was injected intraamniotically. Maternal venous blood was drawn before and at regular intervals for 240 minutes after DHEA-injection. Thereafter, legal abortion was performed by intraamniotic instillation of prostaglandine. The conjugated steroids were hydrolyzed enzymatically and the total steroids were isolated and identified. The following labelled metabolites were determined quantitatively: Estriol (e3, estradiol-17beta (E2-17beta), estrone(E1), 16alpha-hydroxy-estrone, (16alpha-OH-DHEA), ALPHA4-androstenedione (AD) and testosterone (T). The maximal increase of all estrogen fractions in matermal plasma occurred 120-180min after intraamniotic injection of the precursor. The most prominent rise of the C18-steroids could be shown for estriol. 60-70% of all metabolites were C16-hydroxylated. Tritiated-7alpha-dehydroepiandrosterone sulfate (DHEA-S) was injected intraamniotically in 4 women with normal pregnancies between the 18th-20th week of gestation, and its metabolities were determined. Maternal venous blood samples were obtained before and at regular intervals for 4 hours after injection of DHEA-S. Abortion was then induced by intraamniotic instillation of prostaglandins. The following labeled metabolities were identified: estriol, estradiol-17beta, estrone, 16alpha-hydroxy-estrone, dehydroepiandrosterone, delta4-androstenedione and testosterone. All estrogen fractions showed a maximal increase in maternal plasma between 120-180 minutes after injection of DHEA-S. Of the c(18)-steroids, estriol showed the greatest increase. Approximately 60-70% of all the metabolites were c(16)-hydroxylated. The results provide valuable information regarding the quantity of fetoplacental steroid metabolism in midpregnancy.
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