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  • Title: Alteration of RNA I metabolism in a temperature-sensitive Escherichia coli rnpA mutant strain.
    Author: Jung YH, Park I, Lee Y.
    Journal: Biochem Biophys Res Commun; 1992 Aug 14; 186(3):1463-70. PubMed ID: 1380803.
    Abstract:
    E. coli strain A49 carries the themosensitive mutation in the rnpA gene encoding the protein component of RNase P, a tRNA-processing enzyme. Two small RNAs were highly accumulated in the A49 carrying derivatives of ColE1-type plasmids, at nonpermissive temperature. Characterization of these RNAs showed that they were the processed or degraded products derived from RNA I, which is the negative controller of ColE1-type plasmid replication. These derivatives of RNA I only differ in size at the 5' ends. The data of their degradation and synthesis kinetics suggest that they are intermediates of RNA I metabolism.
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