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  • Title: Electron microscope observations on the behavior of the bacterial cytoplasmic membrane during cellular division.
    Author: CHAPMAN GB.
    Journal: J Biophys Biochem Cytol; 1959 Oct; 6(2):221-4. PubMed ID: 13809225.
    Abstract:
    Bacterial cells were fixed in OsO(4), washed, dehydrated, and embedded in a methacrylate mixture. Ultrathin sections were cut on a Porter-Blum ultramicrotome and were examined in an RCA electron microscope, type EMU-2D. The sections revealed that the cytoplasmic membrane undergoes a centripetal growth to form a membrane septum. This septum is formed as a double structure. Constriction of the daughter cells and deposition of cell wall material lead to the separation of the daughter cells. The bacterial cytoplasm appears to consist largely of 200 A granules and occasionally reveals arrays of parallel dense lines.
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