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  • Title: Colocalization of nitric oxide synthase and NADPH-diaphorase in cultured myenteric neurones.
    Author: Saffrey MJ, Hassall CJ, Hoyle CH, Belai A, Moss J, Schmidt HH, Förstermann U, Murad F, Burnstock G.
    Journal: Neuroreport; 1992 Apr; 3(4):333-6. PubMed ID: 1381231.
    Abstract:
    Nitric oxide synthase immunoreactivity and NADPH-diaphorase activity were examined in explant culture preparations of the myenteric plexus from beneath the taenia coli of the guinea-pig caecum. Nitric oxide synthase immunoreactive neurones formed approximately one third of the total neuronal population. NADPH-diaphorase positive neurones, demonstrated histochemically, constituted a similar proportion of the total number of neurones. Immunocytochemistry and NADPH-diaphorase histochemistry performed on the same preparations revealed that all nitric oxide synthase immunoreactive neurones expressed NADPH-diaphorase activity. This histochemical evidence is consistent with the view that nitric oxide may act as a regulatory agent in the guinea-pig caecum.
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