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  • Title: A nonradioactive micro-assay for released reverse transcriptase activity of a lentivirus.
    Author: Cook RF, Cook SJ, Issel CJ.
    Journal: Biotechniques; 1992 Sep; 13(3):380-6. PubMed ID: 1382469.
    Abstract:
    A nonradioactive micro-assay procedure for detection of released reverse transcriptase activity from cells infected with equine infectious anemia virus is described. This procedure utilizes biotinylated-dUTP in conjunction with a streptavidin-alkaline phosphatase conjugate. Detection of alkaline phosphatase is by autoradiography of the chemiluminescence produced during enzymatic dephosphorylation of Lumi Phos 530. This method, as with reverse transcriptase micro-assays employing 32P-labeled nucleotides, is suited to the processing of numerous samples, while having the advantages of safety and stability normally associated with nonradioactive methods of detection. Sensitivity is comparable to a reverse transcriptase micro-assay using 32P-dTTP.
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