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  • Title: [A modified staining method for examining Y chromatin].
    Author: Gao C.
    Journal: Zhongguo Yi Xue Ke Xue Yuan Xue Bao; 1992 Jun; 14(3):229-32. PubMed ID: 1382877.
    Abstract:
    A modified staining method for Y chromatin is reported here which proved to be rapid and efficient for sex differentiation diagnosis. Samples were fixed in methanol: acetic acid (3:1) solution and stained with dyes in McIlvaine buffer. Y chromatin in buccal smears from normal males could be clearly revealed by this method. With this modified method and X-chromatin examination, one testicular feminization was found during examination of 1342 athletes for the 11th Asian Games.
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