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  • Title: Ion channels from the Bacillus subtilis plasma membrane incorporated into planar lipid bilayers.
    Author: Alcayaga C, Venegas R, Carrasco A, Wolff D.
    Journal: FEBS Lett; 1992 Oct 26; 311(3):246-50. PubMed ID: 1383039.
    Abstract:
    Fusion of Bacillus subtilis plasma membrane vesicles with planar lipid bilayers induced the appearance of discrete current fluctuations characteristic of ion channels. These channels showed a wide range of conductances and kinetic behaviors. In 300 mM KCl, their conductances ranged from a few hundreds of pS to more than 1 nS, and most of them exhibited several sub-states. The channels poorly discriminated between small univalent anions and cations. Some of them showed voltage dependence and most of them presented a complex gating kinetics. The results are consistent with the hypothesis of the presence in the B. subtilis plasma membrane of pores composed of subunits that function cooperatively.
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