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  • Title: Detection of endothelin-1 mRNA by RT-PCR in isolated rat renal tubules.
    Author: Uchida S, Takemoto F, Ogata E, Kurokawa K.
    Journal: Biochem Biophys Res Commun; 1992 Oct 15; 188(1):108-13. PubMed ID: 1384475.
    Abstract:
    A combination of reverse transcription and polymerase chain reaction was utilized to detect baseline endothelin-1 (ET-1) mRNA expressed in renal tubules dissected from rats. 5' and 3' primers were constructed according to human ET-1 genomic DNA. Rat ET-1 mRNA was found to be expressed only in cortical through medullary collecting ducts in addition to glomeruli. Sites for tubular synthesis of ET-1 corroborate well with major ET-1 binding sites along the nephron, indicating autocrine/paracrine role of ET-1 in the renal tubules and supporting a prevailing concept on such function of ET-1 in many differing tissues.
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