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  • Title: The absorbance spectrum and photosensitivity of a new synthetic "visual pigment" based on 4-hydroxyretinal.
    Author: Kito Y, Partridge JC, Seidou M, Narita K, Hamanaka T, Michinomae M, Sekiya N, Yoshihara K.
    Journal: Vision Res; 1992 Jan; 32(1):3-10. PubMed ID: 1386953.
    Abstract:
    The firefly squid, Watasenia scintillans, is the only animal known to possess a visual pigment in which the chromophore is 4-hydroxyretinal. This paper describes the absorbance spectrum and some properties of a synthetic "A4" visual pigment generated from bovine opsin and 4-hydroxyretinal. The absorbance spectrum of this pigment is compared with (a) bovine rhodopsin and (b) a rhodopsin template with the same lambda max as the synthetic visual pigment. The A4 pigment is shown to have an absorbance spectrum that is almost identical to that of a rhodopsin template. It is also shown that the photosensitivity and thermal stability of the A4 pigment, dispersed in detergent micelles, is essentially similar to that of rhodopsin.
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