These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Failure of perfusion with oxygenated Krebs-Ringer solution to preserve the eccrine function of the vascular endothelium in bone.
    Author: Moran CG, Wood MB.
    Journal: J Orthop Res; 1992 Nov; 10(6):813-7. PubMed ID: 1403295.
    Abstract:
    An ex vivo canine tibia preparation was perfused at a constant rate with aerated (95% O2-5% CO2) Krebs-Ringer solution for 24 h. Bolus injections of norepinephrine (0.125-0.5 micrograms) were given and then acetylcholine (5 x 10(-5) M) was used to stimulate endothelial production of smooth muscle relaxing factors. Following 1 h of perfusion the addition of acetylcholine resulted in significant attenuation of the response to norepinephrine (p < 0.001). After 4 h perfusion acetylcholine did not attenuate the norepinephrine response, but addition of L-arginine (the precursor of endothelial-derived relaxing factor) resulted in significant attenuation in the presence of acetylcholine (p < 0.005). At 6, 12, and 24 h the acetylcholine did not attenuate the norepinephrine response. It is concluded that normothermic, continuous perfusion with oxygenated Krebs-Ringer solution results in normal endothelial eccrine activity up to 1 h. Following this period there is substrate depletion but endothelial eccrine function can be demonstrated for up to 4 h. At 6 h this function cannot be demonstrated, suggesting degradation of the functional integrity of the endothelium.
    [Abstract] [Full Text] [Related] [New Search]