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  • Title: BROMINE ANALYSIS IN 5-BROMOURACIL-LABELED DNA BY X-RAY FLUORESCENCE.
    Author: ZEITZ L, LEE R.
    Journal: Science; 1963 Dec 27; 142(3600):1670-3. PubMed ID: 14075700.
    Abstract:
    The degree of replacement of thymine by 5-bromouracil in the deoxyribonucleic acid (DNA) of the thymine-deficient bacterium Escherichia coli B 15 T(-) has been determined by a nondispersive x-ray fluorescence method. A measure of the fraction by weight of bromine in the purified DNA is required for determining the degree of thymine replacement. This fraction is experimentally determined on 35-microg samples of DNA by x-ray spectroanalysis.
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