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  • Title: Suppression of tumorigenesis by the breast cancer cell line MCF-7 following transfer of a normal human chromosome 11.
    Author: Negrini M, Castagnoli A, Sabbioni S, Recanatini E, Giovannini G, Possati L, Stanbridge EJ, Nenci I, Barbanti-Brodano G.
    Journal: Oncogene; 1992 Oct; 7(10):2013-8. PubMed ID: 1408142.
    Abstract:
    Breast cancer development is associated with several genetic abnormalities. Loss of heterozygosity in the short arm of chromosome 11 has been observed in 30% of tumors. We found homozygosity at five chromosome 11 polymorphic loci in genomic DNA of the MCF-7 breast carcinoma cell line, suggesting a possible loss of one chromosome 11. We have studied the transformed and tumorigenic phenotypes of MCF-7 cells following introduction of a normal human chromosome 11 via microcell fusion. MCF-7/H11 cell hybrids, containing chromosome 11, showed in vitro characteristics similar to the parental cell line. However, tumorigenicity in athymic mice was completely suppressed. Since tumor formation by MCF-7 cells is estrogen dependent, we have analysed the expression of the estrogen receptor and of the estrogen-activated gene pS2. No difference was detected between the parental MCF-7 cells and the derived chromosome 11 cell hybrids, indicating that the mechanism of MCF-7 tumor suppression by chromosome 11-associated functions does not directly involve the estrogen/estrogen receptor molecular pathway.
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