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  • Title: Unsaturated fatty acid mutants of Neurospora crassa.
    Author: Scott WA.
    Journal: J Bacteriol; 1977 Jun; 130(3):1144-8. PubMed ID: 140860.
    Abstract:
    Unsaturated fatty acid (ufa) auxotrophs of Neurospora crassa were obtained by treatment of conidia with N-methyl-N'-nitro-N-nitrosoguanidine followed by isolation on media containing polyunsaturated fatty acids suspended in Tergitol NP-40. The 24 mutants for which reisolates were obtained from crosses with wild type were assigned to two complementation classes, ufa-1 and ufa-2, located on linkage group V. Unsaturated fatty acids with varying degrees of unsaturation, chain length, and double-bond position as well as different steric configurations were tested for growth requirements.
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