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  • Title: CHEMICALLY DEFINED, SYNTHETIC MEDIA FOR SPORULATION AND FOR GERMINATION AND GROWTH OF BACILLUS SUBTILIS.
    Author: DONNELLAN JE, NAGS EH, LEVINSON HS.
    Journal: J Bacteriol; 1964 Feb; 87(2):332-6. PubMed ID: 14151053.
    Abstract:
    Donnellan, J. Edward, Jr. (U.S. Army Natick Laboratories, Natick, Mass.), Ella H. Nags, and Hillel S. Levinson. Chemically defined, synthetic media for sporulation and for germination and growth of Bacillus subtilis. J. Bacteriol. 87:332-336. 1964.-From 90 to 130 mg (dry weight) of spores (about 1% dark forms) were obtained (per liter) from a chemically defined, synthetic medium, with a two-phase (polyethylene glycol-potassium phosphate) harvest procedure. Optimal sporulation occurred when glucose and glutamic acid were at a concentration of 10 mm in the medium. Ca(++) and Mn(++) were required for sporulation. Heat resistance, dipicolinic acid content, and properties of germination and postgerminative development of spores grown in different concentrations of Ca(++) were investigated. Heat shock did not increase germination of spores derived from the synthetic medium. A synthetic medium, in which spore germination, emergence, and first cell division approached synchrony, was devised.
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