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  • Title: Periodic acid/Schiff staining of glycoproteins immobilized on a blotting matrix.
    Author: Strömqvist M, Gruffman H.
    Journal: Biotechniques; 1992 Nov; 13(5):744-6, 749. PubMed ID: 1418976.
    Abstract:
    A simple and sensitive method for the detection of glycoproteins and glycopeptides in solution and in polyacrylamide gels is described. This method combines the well-known periodic acid/Schiff stain with protein blotting. Compared with direct staining of a polyacrylamide gel, the sensitivity is considerably increased. Using a set of glycoproteins, we have found the sensitivity to be about 4 ng carbohydrate.
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