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  • Title: REPLICATION OF THE RNA OF BACTERIOPHAGE R17.
    Author: FENWICK ML, ERIKSON RL, FRANKLIN RM.
    Journal: Science; 1964 Oct 23; 146(3643):527-30. PubMed ID: 14190242.
    Abstract:
    Escherichia coli cells were irradiated with ultraviolet light to stop ribosomal RNA synthesis. After infection of such cells with the single-stranded RNA phage R17, the RNA most rapidly labeled by a pulse of tritiated uridine sedimented in a broad band in the 16S region of sucrose gradients. The effect of ribonuclease on this material and its behavior during a "chase" period in nonradioactive medium suggest that it consists of a core of double-stranded RNA, one strand of which-the viral strand-is continually displaced by a growing strand forming single-stranded tails and ultimately free 27S viral RNA.
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