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  • Title: Discrimination between alpha-satellite DNA sequences from chromosomes 21 and 13 by using polymerase chain reaction.
    Author: Charlieu JP, Murgue B, Laurent AM, Marçais B, Bellis M, Roizès G.
    Journal: Genomics; 1992 Oct; 14(2):515-6. PubMed ID: 1427870.
    Abstract:
    alpha-Satellite subfamilies from chromosomes 21 and 13 are almost identical in sequence and cannot be distinguished from each other by hybridization techniques. A general method based on membrane-bound PCR is described here, allowing the discrimination of alpha-satellite DNA sequences from each of these two chromosomes, after detection by Southern blot hybridization. The PCR conditions were developed using somatic hybrid DNAs. The method was tested in membrane-bound PCR by using the alpha-satellite bands from a Southern blot of a CEPH family. The chromosomal origin of these bands, previously determined by linkage analysis, was confirmed by this method.
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