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  • Title: STUDIES ON TRANSMISSIBLE GASTROENTERITIS OF SWINE. I. THE ISOLATION AND IDENTIFICATION OF A CYTOPATHOGENIC VIRUS OF TRANSMISSIBLE GASTROENTERITIS IN PRIMARY SWINE KIDNEY CELL CULTURES.
    Author: MCCLURKIN AW.
    Journal: Can J Comp Med Vet Sci; 1965 Feb; 29(2):46-53. PubMed ID: 14290945.
    Abstract:
    Two virus isolates from transmissible gastroenteritis (TGE) of swine were adapted to grow in primary swine kidney cells. Growth of the virus was indicated by the resistance of the infected cells to the cytopathic effect of a virus diarrhea virus of cattle, and by the development of large round cells on the cell sheet. Evidence that these virus isolates were TGE was obtained by the development of signs of the disease followed by death of exposed SPF pigs, or the resistance of the recovered pigs to further signs of disease when they were exposed to virulent TGE contained in virus bearing intestinal tissue. The in vitro and in vivo serum neutralization tests, along with staining of infected cells by fluorescein conjugated TGE antiserum, gave further indication of the specific nature of the virus growing in the cell cultures.
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