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  • Title: MICROBIAL ECOLOGY OF ACTIVATED SLUDGE. II. BACTERIOPHAGES, BDELLOVIBRIO, COLIFORMS, AND OTHER ORGANISMS.
    Author: DIAS FF, BHAT JV.
    Journal: Appl Microbiol; 1965 Mar; 13(2):257-61. PubMed ID: 14325890.
    Abstract:
    A comparative estimation of the coliform population of raw sewage, activated sludge, and the effluent derived therefrom revealed that raw sewage had a preponderance of Escherichia coli (75%), as compared with 25 and 30%, respectively, in sludge and effluent. Nitrogen-free mannitol-sucrose enrichments of activated sludge resulted in the isolation of Azotobacter agilis, Aerobacter aerogenes, Corynebacterium laevaniformans, and an Achromabacter species. Sludge had a large population of C. laevaniformans and A. aerogenes but not of Azotobacter. The bacterial parasites, Bdellovibrio and bacteriophages, were not active during activated-sludge treatment. A 10-fold reduction in phage content occurred after 2 hr of aeration, but the Bdellovibrio population was unaffected.
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