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  • Title: KINETICS OF NICOTINAMIDE NUCLEOTIDE TRANSHYDROGENATION BY HUMAN PLACENTA.
    Author: HAGERMAN DD, VILLEE CA.
    Journal: Biochem J; 1965 Apr; 95(1):150-5. PubMed ID: 14333550.
    Abstract:
    1. Measurements of kinetic constants for a purified preparation of human-placenta oestradiol dehydrogenase have been made. 2. These constants have been compared with similar measurements made on crude ammonium sulphate precipitates of human-placenta homogenates. The comparison indicates that nearly half of the observed nicotinamide nucleotide-transhydrogenation activity in the crude preparations is due to a specific oestrogen-dependent transhydrogenase. 3. The remainder of the observed activity results from a substrate-mediated transhydrogenation catalysed by the oestradiol-dehydrogenase activities present in the preparation.
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