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  • Title: THE SEPARATION OF FERRITIN AND HAEMOSIDERIN FOR STUDIES IN THE METABOLISM OF IRON.
    Author: DRYSDALE JW, RAMSAY WN.
    Journal: Biochem J; 1965 Apr; 95(1):282-8. PubMed ID: 14335815.
    Abstract:
    1. Ferritin and haemosiderin in diluted tissue cannot be quantitatively separated by centrifugation unless the pH is brought below 6. 2. These iron-storage substances can be separated from one another and from haemoglobin by ion-exchange chromatography on CM-cellulose, when good recoveries of total tissue iron are obtained. 3. The ferritin fraction has been identified by its solubility, its appearance under the electron microscope, crystallization with Cd(2+) and the fact that it corresponds quantitatively to the heat-stable portion of the tissue non-haem iron.
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