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  • Title: Isolation of Mycobacterium bovis from the respiratory tracts of skin test-negative cattle.
    Author: Neill SD, Hanna J, Mackie DP, Bryson TG.
    Journal: Vet Rec; 1992 Jul 18; 131(3):45-7. PubMed ID: 1441161.
    Abstract:
    Mycobacterium bovis was isolated from the respiratory tracts of three cattle which registered negative to tuberculin testing; no tuberculous lesions were found and the culture of lymph nodes and other tissues proved negative. One animal was from a group of five calves which had been inoculated intranasally with M bovis, and the organism was recovered once only from nasal mucus sampled 100 days after inoculation. The second animal had had contact with experimentally infected cattle which were excreting M bovis and the third was from a commercial farm. The results of ELISAS for antimycobacterial antibodies and interferon-gamma, and of lymphocyte transformation assays are presented. The animals' immune responses provided evidence that each of them had been challenged.
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