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  • Title: Same-day batch measurement of glycine betaine, carnitine, and other betaines in biological material.
    Author: Lever M, Bason L, Leaver C, Hayman CM, Chambers ST.
    Journal: Anal Biochem; 1992 Aug 15; 205(1):14-21. PubMed ID: 1443552.
    Abstract:
    Glycine betaine, carnitine, carnitine esters, butyrobetaine, and proline betaine (stachydrine) concentrations in biological materials can be reliably measured in 100-microliters samples, with a detection limit below 1 mumol/liter. The procedure is suitable for batches of more than 30 specimens and it is possible to obtain a single result within 2 h. The betaines are extracted into an acetonitrile:methanol mixture, dried with anhydrous disodium hydrogen phosphate containing argentous oxide. The 4-bromophenacyl ester derivatives are formed using 4-bromophenacyl triflate as reagent, in the presence of solid magnesium oxide as base. The derivatives are separated by high-performance chromatography on a silica column, in a mixed partition and ion-exchange mode.
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