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  • Title: [Pepsin in the enzymatic synthesis of esters and n-nitroanilide peptides].
    Author: Lysogorskaia EN, Filippova IIu, Abdel' Malak KA, Lavrenova GI, Anisimova VV, Oksenoĭt ES, Stepanov VM.
    Journal: Bioorg Khim; 1992 Aug; 18(8):1081-8. PubMed ID: 1445435.
    Abstract:
    Pepsin was shown to catalyze synthesis of esters or p-nitroanilides tri-, tetra-, penta- and hexapeptides of general formula Z-X-Y-B, where X = Ala-Phe, Phe-Met, Ala-Ala-Glu, Ala-Ala-Phe, Ala-Ala-Leu, Ala-Ala-Trp, Ala-Ala-Met. Y = Ala, Leu, Val, Phe, Arg, Ala-Ala, Gly-Gly, Leu-Ala-Ala, Phe-Ala-Ala. B = OMe, pNA. The reactions were carried out in dimethylformamide-water solutions at pH 4.6 by equimolar ratio of amino- and carboxyl components (with the exception of Arg-pNA taken in 2-fold excess). The amount of pepsin in the reaction approached 1:1700 enzyme: substrate molar ratio although it might be improved--up to 1:3.10(5) for relatively long peptides.
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