These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Preparation of a fluorescent antibody specific for the yeast phase of Histoplasma capsulatum.
    Author: KAUFMAN L, KAPLAN W.
    Journal: J Bacteriol; 1961 Nov; 82(5):729-35. PubMed ID: 14454457.
    Abstract:
    Kaufman, Leo (Communicable Disease Center, Atlanta, Ga.) and William Kaplan. Preparation of a fluorescent antibody specific for the yeast phase of Histoplasma capsulatum. J. Bacteriol. 82:729-735. 1961.-Rabbit anti-Histoplasma capsulatum globulin was conjugated with fluorescein isothiocyanate. This labeled antibody, in addition to staining six strains of H. capsulatum, exhibited strong cross-staining with ten strains of Blastomyces dermatitidis and numerous other species of fungi. Adsorption of the labeled antiglobulin either twice with cells of B. dermatitidis or twice with cells of Coccidioides immitis and once with cells of B. dermatitidis resulted in a good staining fluorescent antibody reagent, specific for yeast phase cells of H. capsulatum. Attempts to define the specific labeled antibody by the Ouchterlony procedure were unsuccessful. The specific conjugate did not give a complement fixation titer with yeast phase cells of B. dermatitidis or with coccidioidin. It did, however, show a 1:16 complement fixation titer with homologous yeast phase cells and a weaker reaction with histoplasmin.
    [Abstract] [Full Text] [Related] [New Search]